Training of Primary Chicken Monocytes Results in Enhanced Pro-Inflammatory Responses.
Identifieur interne : 000012 ( Main/Exploration ); précédent : 000011; suivant : 000013Training of Primary Chicken Monocytes Results in Enhanced Pro-Inflammatory Responses.
Auteurs : Michel B. Verwoolde [Pays-Bas] ; Robin H G A. Van Den Biggelaar [Pays-Bas] ; Jürgen Van Baal [Pays-Bas] ; Christine A. Jansen [Pays-Bas] ; Aart Lammers [Pays-Bas]Source :
- Veterinary sciences [ 2306-7381 ] ; 2020.
Abstract
Beta-glucan-stimulated mammalian myeloid cells, such as macrophages, show an increased responsiveness to secondary stimulation in a nonspecific manner. This phenomenon is known as trained innate immunity and is important to prevent reinfections. Trained innate immunity seems to be an evolutionary conserved phenomenon among plants, invertebrates and mammalian species. Our study aimed to explore the training of primary chicken monocytes. We hypothesized that primary chicken monocytes, similar to their mammalian counterparts, can be trained with β-glucan resulting in increased responses of these cells to a secondary stimulus. Primary blood monocytes of white leghorn chickens were primary stimulated with β-glucan microparticulates (M-βG), lipopolysaccharide (LPS), recombinant chicken interleukin-4 (IL-4) or combinations of these components for 48 h. On day 6, the primary stimulated cells were secondary stimulated with LPS. Nitric oxide (NO) production levels were measured as an indicator of pro-inflammatory activity. In addition, the cells were analyzed by flow cytometry to characterize the population of trained cells and to investigate the expression of surface markers associated with activation. After the secondary LPS stimulation, surface expression of colony stimulating factor 1 receptor (CSF1R) and the activation markers CD40 and major histocompatibility complex class II (MHC-II) was higher on macrophages that were trained with a combination of M-βG and IL-4 compared to unstimulated cells. This increased expression was paralleled by enhanced NO production. In conclusion, this study showed that trained innate immunity can be induced in primary chicken monocytes with β-glucan, which is in line with previous experiments in mammalian species. Innate immune training may have the potential to improve health and vaccination strategies within the poultry sector.
DOI: 10.3390/vetsci7030115
PubMed: 32825152
PubMed Central: PMC7560005
Affiliations:
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Verwoolde</name><affiliation wicri:level="1"><nlm:affiliation>Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen</wicri:regionArea><wicri:noRegion>6708 WD Wageningen</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Animal Nutrition Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Animal Nutrition Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen</wicri:regionArea><wicri:noRegion>6708 WD Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Van Den Biggelaar, Robin H G A" sort="Van Den Biggelaar, Robin H G A" uniqKey="Van Den Biggelaar R" first="Robin H G A" last="Van Den Biggelaar">Robin H G A. Van Den Biggelaar</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Van Baal, Jurgen" sort="Van Baal, Jurgen" uniqKey="Van Baal J" first="Jürgen" last="Van Baal">Jürgen Van Baal</name><affiliation wicri:level="1"><nlm:affiliation>Animal Nutrition Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Animal Nutrition Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen</wicri:regionArea><wicri:noRegion>6708 WD Wageningen</wicri:noRegion></affiliation></author><author><name sortKey="Jansen, Christine A" sort="Jansen, Christine A" uniqKey="Jansen C" first="Christine A" last="Jansen">Christine A. Jansen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Lammers, Aart" sort="Lammers, Aart" uniqKey="Lammers A" first="Aart" last="Lammers">Aart Lammers</name><affiliation wicri:level="1"><nlm:affiliation>Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen</wicri:regionArea><wicri:noRegion>6708 WD Wageningen</wicri:noRegion></affiliation></author></analytic><series><title level="j">Veterinary sciences</title><idno type="eISSN">2306-7381</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Beta-glucan-stimulated mammalian myeloid cells, such as macrophages, show an increased responsiveness to secondary stimulation in a nonspecific manner. This phenomenon is known as trained innate immunity and is important to prevent reinfections. Trained innate immunity seems to be an evolutionary conserved phenomenon among plants, invertebrates and mammalian species. Our study aimed to explore the training of primary chicken monocytes. We hypothesized that primary chicken monocytes, similar to their mammalian counterparts, can be trained with β-glucan resulting in increased responses of these cells to a secondary stimulus. Primary blood monocytes of white leghorn chickens were primary stimulated with β-glucan microparticulates (M-βG), lipopolysaccharide (LPS), recombinant chicken interleukin-4 (IL-4) or combinations of these components for 48 h. On day 6, the primary stimulated cells were secondary stimulated with LPS. Nitric oxide (NO) production levels were measured as an indicator of pro-inflammatory activity. In addition, the cells were analyzed by flow cytometry to characterize the population of trained cells and to investigate the expression of surface markers associated with activation. After the secondary LPS stimulation, surface expression of colony stimulating factor 1 receptor (CSF1R) and the activation markers CD40 and major histocompatibility complex class II (MHC-II) was higher on macrophages that were trained with a combination of M-βG and IL-4 compared to unstimulated cells. This increased expression was paralleled by enhanced NO production. In conclusion, this study showed that trained innate immunity can be induced in primary chicken monocytes with β-glucan, which is in line with previous experiments in mammalian species. Innate immune training may have the potential to improve health and vaccination strategies within the poultry sector.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32825152</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2306-7381</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>Aug</Month><Day>19</Day></PubDate></JournalIssue><Title>Veterinary sciences</Title><ISOAbbreviation>Vet Sci</ISOAbbreviation></Journal><ArticleTitle>Training of Primary Chicken Monocytes Results in Enhanced Pro-Inflammatory Responses.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E115</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/vetsci7030115</ELocationID><Abstract><AbstractText>Beta-glucan-stimulated mammalian myeloid cells, such as macrophages, show an increased responsiveness to secondary stimulation in a nonspecific manner. This phenomenon is known as trained innate immunity and is important to prevent reinfections. Trained innate immunity seems to be an evolutionary conserved phenomenon among plants, invertebrates and mammalian species. Our study aimed to explore the training of primary chicken monocytes. We hypothesized that primary chicken monocytes, similar to their mammalian counterparts, can be trained with β-glucan resulting in increased responses of these cells to a secondary stimulus. Primary blood monocytes of white leghorn chickens were primary stimulated with β-glucan microparticulates (M-βG), lipopolysaccharide (LPS), recombinant chicken interleukin-4 (IL-4) or combinations of these components for 48 h. On day 6, the primary stimulated cells were secondary stimulated with LPS. Nitric oxide (NO) production levels were measured as an indicator of pro-inflammatory activity. In addition, the cells were analyzed by flow cytometry to characterize the population of trained cells and to investigate the expression of surface markers associated with activation. After the secondary LPS stimulation, surface expression of colony stimulating factor 1 receptor (CSF1R) and the activation markers CD40 and major histocompatibility complex class II (MHC-II) was higher on macrophages that were trained with a combination of M-βG and IL-4 compared to unstimulated cells. This increased expression was paralleled by enhanced NO production. In conclusion, this study showed that trained innate immunity can be induced in primary chicken monocytes with β-glucan, which is in line with previous experiments in mammalian species. Innate immune training may have the potential to improve health and vaccination strategies within the poultry sector.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Verwoolde</LastName><ForeName>Michel B</ForeName><Initials>MB</Initials><Identifier Source="ORCID">0000-0003-3630-8106</Identifier><AffiliationInfo><Affiliation>Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Animal Nutrition Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van den Biggelaar</LastName><ForeName>Robin H G A</ForeName><Initials>RHGA</Initials><Identifier Source="ORCID">0000-0001-6247-9705</Identifier><AffiliationInfo><Affiliation>Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>van Baal</LastName><ForeName>Jürgen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Animal Nutrition Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jansen</LastName><ForeName>Christine A</ForeName><Initials>CA</Initials><Identifier Source="ORCID">0000-0003-0740-0835</Identifier><AffiliationInfo><Affiliation>Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lammers</LastName><ForeName>Aart</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-1876-9630</Identifier><AffiliationInfo><Affiliation>Adaptation Physiology Group, Department of Animal Sciences, Wageningen University and Research, De Elst 1, 6708 WD Wageningen, The Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>868.15.030</GrantID><Agency>Nederlandse Organisatie voor Wetenschappelijk Onderzoek</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Vet Sci</MedlineTA><NlmUniqueID>101680127</NlmUniqueID><ISSNLinking>2306-7381</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">flow cytometry</Keyword><Keyword MajorTopicYN="N">inflammatory response</Keyword><Keyword MajorTopicYN="N">innate immune memory</Keyword><Keyword MajorTopicYN="N">macrophages</Keyword><Keyword MajorTopicYN="N">primary chicken monocytes</Keyword><Keyword MajorTopicYN="N">β-glucan</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>07</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>08</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>08</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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(province)</li></region><settlement><li>Utrecht</li></settlement><orgName><li>Université d'Utrecht</li></orgName></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Verwoolde, Michel B" sort="Verwoolde, Michel B" uniqKey="Verwoolde M" first="Michel B" last="Verwoolde">Michel B. Verwoolde</name></noRegion><name sortKey="Jansen, Christine A" sort="Jansen, Christine A" uniqKey="Jansen C" first="Christine A" last="Jansen">Christine A. Jansen</name><name sortKey="Lammers, Aart" sort="Lammers, Aart" uniqKey="Lammers A" first="Aart" last="Lammers">Aart Lammers</name><name sortKey="Van Baal, Jurgen" sort="Van Baal, Jurgen" uniqKey="Van Baal J" first="Jürgen" last="Van Baal">Jürgen Van Baal</name><name sortKey="Van Den Biggelaar, Robin H G A" sort="Van Den Biggelaar, Robin H G A" uniqKey="Van Den Biggelaar R" first="Robin H G A" last="Van Den Biggelaar">Robin H G A. Van Den Biggelaar</name><name sortKey="Verwoolde, Michel B" sort="Verwoolde, Michel B" uniqKey="Verwoolde M" first="Michel B" last="Verwoolde">Michel B. Verwoolde</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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